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Entry from: ICLC
 
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CABRI:ICLC
Accession_number ICLC HTL12001
Cell_line_name 92.1
Brief_description Species: human; Tissue: uvea; Tumor: melanoma
Description Species: human female; Tissue: uvea; Tumor: melanoma
Depositor Dr U. Pfeffer, Molecular Pathology Integrated , IRCCS AOU San Martino-IST, Genoa Italy
Bibliographic_reference -
Morphology epithelial, grown as monolayer
Culture_conditions continuous culture; RPMI 1640 + 10% FBS + 2mM L-Glutamine; 37C, 5% CO2, split cultures 1:2-1:5 using trypsin/EDTA
Viruses Search for viruses was not performed
Properties melanin production
Release_conditions Cell line available for distribution. For non-commercial investigative use only
Hazard -
Freezing_medium 90% FBS + 10% DMSO
Sterility mycoplasma negative, HOECHST and PCR
Validation_assays DNA Profile STR (Short Tandem Repeat)
Comments Cell line originator: Sarah Coupland
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